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Course Outline
Introduction to Fiji and ImageJ
- An overview of Fiji and its utility in scientific research
- Exploring the architecture of ImageJ and its plugin ecosystem
- Installing and configuring Fiji for optimal performance
Basic Image Processing Techniques
- Image acquisition and supported import formats
- Modulating contrast and brightness, and applying digital filters
- Image stacking and various projection methods
Advanced Image Analysis
- Segmentation methodologies, including thresholding and edge detection
- Counting objects and performing particle analysis on histological samples
- Measuring areas, perimeters, and other morphological characteristics
Working with Biological Samples
- Processing images of histological tissues, blood cells, and algae
- Colour deconvolution and analysis of stained tissue samples
- Time-lapse analysis and tracking cellular movement
Automation and Customization
- Developing and executing macros for repetitive tasks
- Utilising Fiji plugins for specialised analytical procedures
- Introduction to scripting with the ImageJ Macro Language (IJM)
Data Management and Reporting
- Exporting results and compiling comprehensive reports
- Annotating images and structuring data for publication
- Best practices for ensuring reproducible image analysis
Requirements
- A foundational understanding of biological imaging principles
- Knowledge of microscopy and histological methodologies
- General computer proficiency
Target Audience
- Researchers specialising in biotechnology and environmental toxicology
- Laboratory technicians engaged in histological and cellular imaging
- Scientists seeking to master quantitative image analysis
14 Hours
Custom Corporate Training
Training solutions designed exclusively for businesses.
- Customized Content: We adapt the syllabus and practical exercises to the real goals and needs of your project.
- Flexible Schedule: Dates and times adapted to your team's agenda.
- Format: Online (live), In-company (at your offices), or Hybrid.
Price per private group, online live training, starting from 3200 € + VAT*
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